84-1016-36　Green Fluorescent Protein (GFP) 200ug　G8965-06

特徴

• Green fluorescent protein (GFP) is a 29kD fluorescent protein isolated from the jellyfish Aequorea victoria.
• Upon exposure to blue light (488nm), GFP strongly fluoresces (Emission max: 507nm).
• GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells
• Applications:Suitable for use in Western Blot and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 0.5-2ug/ml detects Green Fluorescent Protein from 50ug of rat brain lysate containing 25ng of purified Green Fluorescent Protein.
• Also detected a GFP-fusion protein from a transfected E.
• coli cell lysate.
• Rat brain lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8965-06 (1ug/ml).
• Proteins were visualized using a rabbit anti-chicken secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunocytochemistry: Reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
• Positive Control:rat brain lysate, cells expressing Green Fluorescent Protein
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.