84-1016-33　IRAK (IL-1 Receptor-associated Kinase) 200ug　I8600-05

特徴

• The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP).
• IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response.
• A novel member in the IRAK/Pelle family was recently identified and designated IRAK2.
• Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation.
• IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation.
• Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory responses to cytokine IL-1 and LPS.
• IRAK2 is expressed in a variety of human tissues.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/ml detects IRAK in HeLa nuclear extract.
• HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with I8600-05 (1ug/ml).
• Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
• Immunoprecipitation: 4ug immunoprecipitates IRAK from 500ug of HeLa nuclear extract.
• Optimal dilutions to be determined by the researcher.
• Positive Control Included:H1838: HeLa nuclear extract.
• Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation.
• Load 10- 20ug/lane for minigels.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.