84-1016-31　［取扱停止］STAT 5a,b, phosphorylated (Ser726/Ser731) (Signal Transducer and Activator of Transcription 5a,b) 200ul　S7972-12

特徴

• Many hormones and cytokines selectively activate transcription factors of the STAT gene family.
• STAT5 was originally cloned as a prolactin-stimulated DNA-binding protein, but is also activated by non-lactogenic cytokines in many cell types.
• Phosphorylation of a conserved tyrosine residue is required for STAT dimerization, nuclear translocation, and specific DNA binding.
• Serine residues located in a conserved PSP motif of STAT5A (S726) and STAT5B (S731) are also major phosphorylation sites.
• Interestingly, control of the phosphorylation of the PSP motif differs between the STAT5A (S725) and STAT5B (S730) serine sites.
• Applications:Suitable for use in Immunoprecipitation/Western Blot.
• Other applications have not been tested.
• Recommended Dilutions:Immunoprecipitation/Western Blot Analysis: 1:500-1:2000 detected phosphorylated STAT5A following immunoprecipitation of STAT5A from RIPA lysates of Nb2-Sp cells that had been treated with prolactin; 4ul of S7972-10 was used to immunoprecipitate total STAT5A.
• Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
• Phosphospecificity was confirmed by dephosphorylation of the STAT5a protein with l-phosphatase
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.