Spinophilin/Neurabin-II has been found to be enriched at the postsynaptic density fraction in the brain and in the adherens junction fraction in rat liver.
Within the brain, Spinophilin/Neurabin-II is localized in the heads of dendritic spines.
Spinophilin/Neurabin-II contains a single PDZ domain in the middle region of the protein and other domains that predict it will form a coiled-coil structure at the C-terminus.
Spinophilin/Neurabin-II is known to complex with the catalytic subunit of protein phosphatase I (PP1) and in vitro is a potent modulator of PP1 enzymatic activity.
Spinophilin/Neurabin-II is also known to interact with F-actin and rat lin-10.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.25-1ug/ml detects Spinophilin in 20ug of a rat brain microsomal preparation.
Immunoprecipitation: 4ug immunoprecipitates Spinophilin from 500ug of rat brain microsomal preparation
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, pH 7.4, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes rat Spinophilin at ~120kD. Also recognizes a band at ~95kD which is suspected to be a cleavage product of spinophilin.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Rt
Immunogen：GST fusion protein corresponding to residues 286-390 of rat brain Spinophilin.
