Calreticulin was originally identified as a Ca2+ binding protein in the muscle sarcoplasmic reticulum.
Since its original discovery, it has been found in high abundance in non-muscle tissue.
Calreticulin has the ability to bind Ca2+ with high capacity but low affinity.
The exact function of calreticulin is not yet known, however recent reports suggest that it may play a role in nuclear hormone receptor gene transcription and may also act as a chaperone protein.
Calreticulin is 90% conserved among human, mouse, rat, rabbit and Aplysia.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml detects calreticulin in RIPA lysates from murine 3T3/A31 cells.
3T3/A31 cell lysates were resolved by electrophoresis, transferred to PVDF and probed with C1036 (0.5ug/ml).
Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system
Immunohistochemistry: Detects calreticulin in tissue sections and cells.
Optimal diltions to be determined by the researcher.
Included Positive Antigen Control:F4209: Fibroblast Cell, 3T3/A31 lysate.
Add 2.5ul 2-mercaptoethanol/100ul of lysate.
Boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for Western Blot analysis.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a frozen solution in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human calreticulin. Does not crossreact with other endoplasmic reticulum proteins. Species Crossreacitivity: mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Po Rt Xe
Immunogen：Synthetic peptide corresponding aa412-417, QAKDEL, at the C-terminus of human calreticulin, conjugated to KLH.
