84-1016-16　Histone H1, phosphorylated 200ug　H5110-03

特徴

• Histones are a family of basic proteins found in all eukaryotic nuclei that are associated with eukaryotic DNA.
• The five major types of histone are H1, H2A, H2B, H3 and H4.
• The latter four, which comprise nucleosome core, are evolutionarily conserved, while the linker histone H1 exhibits greater sequence diversity among organisms.
• The amino acid sequence of H1 varies more from organism to organism.
• The separation of H1 into multiple bands by gel electrophoresis results from differences in the extent of phosphorylation of residues in the protein.
• Applications:Suitable for use in Western Blot and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/ml detected phosphorylated Histone H1 from acid extracts of colcemid treated HeLa cells.
• Does not detect Histone H1 in untreated HeLa cells.
• Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-03 (2ug/ml).
• Proteins were visualized using an IgG (HRP) goat anti-rabbit.
• A chemiluminescence detection system was used.
• Immunocytochemistry: 5-10ug/ml showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95% ethanol, 5% glacial acetic acid
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.