The lymphocyte-specific protein tyrosine kinase Lck is a member of the src family of nonreceptor tyrosine kinases.
Abundant expression of Lck in normal and transformed T-cells, as well as in lymphoid tissue, suggests that Lck plays a role in lymphoid cell growth and differentiation.
Furthermore, Lck is associated with interleukin receptors and is involved during Epstein-Barr virus infection leading to lymphocyte immortalization.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5–2ug/ml detected Lck in RIPA lysates from Jurkat cells.
Immunoprecipitation: 4ug immunoprecipitated Lck from 1ug of Jurkat RIPA lysate as confirmed by subsequent immunoblot using monoclonal anti-Lck.
Immunoblot Analysis Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Lck (1ug/ml).
Proteins were visualized using a goat anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:Jurkat cell lysate.
Use 20ug per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a solution in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes Lck, Mr 56kD of mouse, human and bovine; reactivity with other Src family members not determined.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Bo Hu Mo
Immunogen：GST fusion protein corresponding to aa1-58 of human Lck.
