The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3).
Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family.
Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer.
ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas.
Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500 detects c-erbB-2 on 10ug of HEK293 lysates
Immunohistochemistry: Reported to detect c-erbB-2 in frozen and formalin/paraffin sections of rat breast.
Immunoprecipitation: 4ug immunoprecipitated c-erbB-2 from 600ug of MCF-7 RIPA lysate and previously in A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human c-erbB-2 (185kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4. Species Crossreactivity: monkey and mouse
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：KLH-conjugated synthetic peptide corresponding to AENPEYLGLDVPV aa1243-1255 from human c-erbB-2/HER-2/neu oncoprotein. Species Sequence Homology: rat
