84-1016-07　FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) 200ug　F0019-56

特徴

• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Immunocytochemistry: 10ug/ml shows positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde
• Western Blot: 0.5-2ug/ml detects FAK in RIPA lysates from murine 3T3 and previously in human A431 RIPA cell lysate.
• 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with F0019-56 (1ug/ml).
• Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
• Immunoprecipitation: 4ug immunoprecipitates FAK from 500ug of murine 3T3 RIPA lysate.
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRbI1904-40D: IgG, H&L (FITC) Pab Gt xRbI1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb I1904-46N: IgG, H&L (FITC) Pab Gt xRb
• Positive Antigen Control: F42093T3 cell lysate; use 20mg per lane for minigels.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.