Na+/K+ ATPase a-1 is a human gene and functions to maintain Na+ and K+ across the cell membrane which is important for osmoregulation.
It is a component of (Na+/K+) ATPase, a transporter that catalyzes the hydrolysis of ATP and transports sodium and potassium ions across the plasma membrane.
It also partners with Src and other signaling proteins.
Binding of ouabain and other cardiotonic steroids to the (Na+/K+) ATPase activates Src, resulting in the assembly and activation of multiple signaling cascades.
Binding of ouabain also induces a positive inotropic effect.
Diseases associated with ATPase are Thyrotoxic periodic paralysis and hyperthyroidism.
Applications:Suitable for use in Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunoprecipitation: 10ug immunoprecipates ATPase from 500ug of rat brain microsomal lysates
Immunohistochemistry: 1:500Optimal dilutions to be determined by the researcher.
Positive Control:Rat Brain Microsomal Prep.
lysate.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes rat ATPase a-1 subunit isoform at Mr of 110kD. Species Crossreactivity: human and bovine.
