Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506.
Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP).
Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506.
Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC.
CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000-1:10,000 detects Cyclophilin A in RIPA lysates from nonstimulated A431 cells.
A previous lot detected Cyclophilin A in RIPA lysates from rat lung cells.
20ug of A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8850 (1:10,000).
Proteins were visualized using a Gt xRb IgG (HRP)* and a chemiluminescence detection system
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:A0001-50: Non stimulated A431 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes human cyclophilin A (18kD). Additional bands may be detected at higher concentrations of the antibody. Species Crossreactivity: rat, mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length protein corresponding to human cyclophilin A.
