84-1016-01　［取扱停止］Platelet Derived Growth Factor Receptor Type B (PDGFRb) 200ug　P4258-23

特徴

• Two types of PDGF receptors have been identified: PDGF receptor A (PDGFRa) and PDGF receptor B (PDGFRb).
• The PDGFRa, a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGFRb, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity.
• Both receptors are transmembranous, ligand-activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains.
• The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/ml detects PDGFb in F4209.
• F4209 was resolved by electrophoresis, transferred to nitrocellulose and probed with P4258-23 (2ug/ml).
• Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
• Immunoprecipitation: 5ug immunoprecipitates PDGFb in F4209
• Optimal dilutions to be determined by the researcher.
• Positive Antigen Control Included:F4209: 3T3/A31 cell lysate.
• Use 20ug/lane for minigels.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.