84-1015-96　［取扱停止］MAP Kinase 2 (Mitogen-activated Protein Kinase 2, MAPK 2, MAPK2, Extracellular Signal-regulated Kinase 2, ERK2, ERK-2, ERK, ERT1, MAP Kinase Isoform p42, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, Mapk1, Mapk, p42mapk, p42-MAPK, Prkm1, PRKM2) 200ug　M2362-05

特徴

• ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines.
• Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK.
• ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord.
• Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommneded Dilutions:Western Blot: 0.5-2ug/ml detected p42MAP Kinase (erk2) in human A431 carcinoma cells, mouse 3T3/A31 fibroblasts and rat L6 skeletal fibroblasts.
• Immunoprecipitation: 5ug of this antibody immunoprecipitated p42MAPK (erk2) from 3T3/A31 cells as confirmed by Western Blot using M2362
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMoI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
• Positive Control Included:F4209: Fibroblast Cell, 3T3/A31Add 2.5ul 2-mercaptoethanol/100ul of lysate.
• Boil for 5 minutes to reduce the preparation.
• Load 20ug of reduced lysate per lane for Western Blot analysis.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.