84-1015-91　Nitrotyrosine (nTyr) 100ug　N2700-06

特徴

• Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO.
• It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell.
• Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.
• Applications:Suitable for use in Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/ml detects nitrotyrosine immunoblot controls N2705 at 16kD, 66kD and 215kD, corresponding to nitrated superoxide dismutase, nitrated BSA, and nitrated rabbit muscle myosin, respectively.
• An ~32kD band might be seen representing a dimer of the 16kD protein.
• Nitrotyrosine immunoblot controls were resolved by electrophoresis, transferred to nitrocellulose and probed with N2700-06 (2ug/ml).
• Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
• Immunohistochemistry: 5ug/ml detects nitrated proteins in formalin-fixed paraffin embedded lateral ischemic rat brain tissue.
• Has been reported to detect nitrotyrosine-containing proteins in acutely injured human lung tissue
• Immunocytochemistry: 5-10ug/ml detects nitrated proteins from human A431 cells pretreated 5 minutes with 5-10mM peroxynitrite
• Optimal dilutions to be determined by the researcher.
• Positive Controls:Immunohistochemistry: Human Alzheimer disease brains sections (Methacarn fixed) and mouse liverWestern Blot: Nitrated-BSA
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.