Gi alpha 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-gamma activation and Ca+2 mobilization in hepatocytes1,2.
G-proteins are membrane associated heterotrimeric proteins that are comprised of alpha, beta-, and lambda-subunits.
The alpha-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP.
Upon activation, GDP is replaced with GTP, causing the dissociation of the alpha-subunit from the beta, lambda-subunit complex.
This enables the G alpha-GTP complex to bind to and regulate specific signaling pathways.
GTP is then hydrolyzed, allowing for re-association of the alpha-subunit with the beta, lambda- subunit complex.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:2000 detected Gi alpha 3 in rat hippocampus membrane preparation.
Previous lots detected Gi apha 3 in solubilized rat and mouse brain membranes and mouse 3T3/A31 cell lysates
Immunoprecipitation: 4ul immunoprecipitated Gi alpha 3 from a solubilized mouse brain membrane lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.4.
Specificity：Recognizes Gi alpha 3 and Go alpha, Mr 40kD. Low crossreactivity with other G-proteins. Species Crossreactivity: human, mouse, hamster and rat
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hm Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 10aa, KNNLKECGLY from the C-terminal region of Gi alpha 3.
