The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase.
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals.
This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals.
As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot: 0.5-2.0ug/ml detects MEK1 in RIPA lysates from A431 and 3T3/A31 cells.
A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M2865-07 (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
MEK1 (~45kD).
Immunoprecipitation: 4ug immunoprecipitates MEK1 from 500ug of A431 and 3T3/A31 RIPA lysate.
MEK1 Immunoprecipitation Kinase Assay, nonradioactive:4ug of this lot immunoprecipitated active MEK1 from NGF stimulated PC-12 cell lysates, as well as active human MEK1
Optimal dilutions to be determined by the researcher.
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 30% glycerol.
Specificity：Recognizes mouse MEK1 at 45kD. Species Crossreactivity: Human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa2-18 (CPKKKPTPIQLNPAPDGS) of mouse Map Kinase Kinase (MEK1)(KLH) Species sequence homology: rat, mouse, rabbit-100%; Chinese hamster and chicken-16/17.
