84-1015-88　IRS-1 (Insulin Receptor Substrate 1) 100ug　I8700

特徴

• IRS-1 is one of the major substrates of insulin receptor kinase.
• IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain.
• IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain–containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growthpromoting functions of insulin.
• IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK.
• Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/ml detects IRS-1 in RIPA lysates from 3T3/A31 mouse fibroblasts.
• 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with I8700 (0.5ug/ml).
• Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts.
• In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5ug/ml
• Immunoprecipitation: 4ug immunoprecipitates IRS-1 from 500ug of 3T3/A31 RIPA lysateOptimal dilutions to be determined by the researcher.
• Positive Control:Fibroblast Cell, 3T3/A31, L6 myoblasts.
• Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.