84-1015-85　Gi alpha 1,2 50ul　G2033-90

特徴

• Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
• The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000 detected the Gia-1 and Gia-2 protein in 40ug rat brain microsomal preparation and in 10ug of solubilized plasma membranes from mouse 3T3/A31 fibroblasts.
• The rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G2033-90 (1:1000).
• Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
• Immunocytochemistry: 1:500 dilution gave positive immunostaining of mouse 3T3/A31 fibroblasts fixed for 3 minutes with ice-cold 100% acetone
• Optimal dilution determined by the researcher.
• Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRbI1904-40D: IgG, H&L (FITC) Pab Gt xRbI1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.