84-1015-83　c-Jun (Activator protein 1, AP1, p39, Proto-oncogene c-Jun, Transcription factor AP-1, V-jun avian sarcoma virus 17 oncogene homolog) 200ul　C5815

特徴

• c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway.
• The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73.
• Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription.
• Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways.
• The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73.
• In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:2000 detected c-Jun in RIPA lysates from human A431 cells, and previously in Jurkat cell lysate.
• A431 RIPA cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C5815 (1:1000ug/ml).
• Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunoprecipitation: 5ulImmunocytochemistry: 1:200 Optimal dilutions to be determined by the researcher.
• Positive Control (A0001-50):Non-stimulated A431 cell lysate.
• Use 10-20ug per lane.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.