Platelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans.
The biologically active protein is a dimer composed of two related polypeptides designated A and B.
The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.
Applications:Suitable for use in Western Blot and Growth Inhibition Assays.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 5-10ug/ml, under non-reducing conditions, detects 100ng of human PDGF-AB (MW~28kD) as well as 100ng of human PDGF-BB (MW~30-32kD).
Growth Inhibition Assay: In a 5 day assay, 10-20ug/ml inhibited 3T3 cell growth by 100% as stimulated by 10ng/ml of PDGF-AB.
Opimal dilutions to be determined by the researcher.
Growth Inhibition Assay Protocol:1.
In DMEM, 1% calf serum, preincubate PDGF-AB (10ng/ml) and P4258-19, PDGF (10-20ug/ml) for one hour at 37°C.
Serially dilute P4258-19 2-fold from 40ug/ml to 2.5ug/ml in a 96 well flat black view plate
2.
Prepare a 3T3 cell suspension at 10e4 cells/ml and aliquot 100ul to each well
3.
Refeed on Day 3
4.
Count wells on day 5 using an ATP endpoint assay.
Wash cell layer once with PBS prior to lysis.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.0.
Specificity：Recognizes non-reduced human PDGF AA, AB and BB dimers in Western Blot. Porcine and bovine PDGF may be detected with decreased sensitivity. Rodent PDGF will not be detected. This antibody will neutralize human, porcine, bovine and rodent PDGF.
