84-1015-75　［取扱停止］SHP 2 (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11) 200ug　S1013-29

特徴

• SHP2 is a ubiquitously expressed tyrosine-specific protein phosphatase.
• It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death.
• Activation of SHP 2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.
• In addition to its role in Gab1-mediated Erk activation, SHP 2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 1-4ug/ml detects SHP 2/SHPTP-2 in RIPA lysates from human A431 carcinoma cells and mouse 3T3/A31 fibroblasts.
• Strongly recognizes the SHP 2/SHPTP-2 fusion protein (10ng)Immunoprecipitation: 6ug immunoprecipitates SHP 2/SHPTP-2 from RIPA lysates of human A431 carcinoma cells.
• An immunoprecipitation phosphatase assay using phenylphosphate as a substrate was performed using EGF stimulated A431 cells
• Immunocytochemistry: 10ug/ml detects SHP 2/SHPTP-2 in EGF stimulated A431 cells
• Neutralization: Does not inhibit phosphotyrosine phosphatase activity.
• Optimal dilutions to be determined by the researcher.
• Positive Antigen Control Included:A0001-50: Non stimulated A431 cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.