IGFBP-1 (Insulin-like Growth Factor Binding Protein-1) is produced by the liver and regulated by glucocorticoids and insulin at the level of gene transcription.
The N-terminal and C-terminal regions are highly homologous among rat, human and bovine sequences.
IGFBP-1 inhibits IGF binding to cell surface receptors and thereby inhibits IGF-mediated mitogenic and cell metabolic actions.
Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000 detects 10ng SDS-denatured human IGFBP-1
Immunoprecipitation: 1:500 precipitates >90% of 10ng IGFBP-1.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes human IGFBP-1 at 30kD. Slight crossreactivity (<1%) with IGFBP-2, -3, -4, -5. Does not crossreact with bovine, equine, goat or sheep IGFBP-1. Crossreacts weakly with porcine IGFBP-1.
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Hu
Immunogen：IGFBP-1 purified from human amniotic fluid.
