Type IV collagen is a major component of the basement membrane and it associates with laminin, entactin and heparin sulfate proteoglycans to form sheet-like structures that separates epithelium from connective tissue.
It is a long triple helix containing several interruptions, which is characterized by a specific carboxylterminal, non-collagenous NC1 domain.
It is composed of six genetically different alpha (IV) chains, alpha1 (IV) to alpha 6(IV) and their genes are paired on three different chromosomes in a head-to-head arrangement.
The alpha 1 gene and the alpha 2 gene are localized on chromosome 13q34, the alpha 3 gene and the alpha 4 gene are on chromosome 2q36, and the alpha 5 gene and the alpha 6gene are on chromosome Xq22.
Type IV collagen can be used as a thin coating on tissue culture surfaces to promote cell attachment and proliferation and to study its effects on cell behavior.
Applications:Suitable for use in Immunofluorescence, Immunohistochemistry and ELISA.
Other applications have not been tested.
Recommended Dilution:Immunofluorescence: 1:40-1:160Immunohistochemistry (paraffin): 1:250-1:1000 (Recommended Immunohistochemistry fixatives: AFA, buffered formalin (5-10%) or Bouin’s)ELISA: 1:100-1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromotography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes mouse collagen Type IV: 100%; mouse Types I, II, III: <0.1%; Human Types IV, V: <0.1%; Mouse Fibronectin: <0.1%; Mouse Laminin: <0.1%. Determined by RIA. Species Crossreactivity: rat and orangutan, reacts weakly with human.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Mo Rt
Immunogen：Native Collagen Type IV from mouse EHS tumor.
