A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively, (1-3).
RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain.
Overexpression of RICK induced apoptosis and activation of NF-kB and JNK.
RICK interacts with members of the TRAF family, CLARP and caspase-1.
Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1b.
The messenger RNA of RICK is expressed in multiple human tissues (1).
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500Immunocytochemistry: 5ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control: K562 or 3T3 whole cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human RICK. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：15aa peptide near the C-terminal of human RICK.
