Glial cell line-derived neurotrophic factor (GDNF) plays key roles in the control of vertebrate neuron survival and differentiation.
A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin (1, 2).
Physiological responses to neurturin (NTN) require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRa (3,4).
The cDNAs encoding NTNRa from human, rat, chicken, and mouse have been cloned recently (3-8) and termed GDNFRb, Ret ligand 2 (RETL2) or TGF-b-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRa-2 recently (9).
NTN binds to and forms a complex with GFRa-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway.
Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations.
Neurturin and GDNF define a new family of neurotrophic factors.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000.
An approximate 14kD band of the full length recombinant NTN was detected.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%) aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Immunoaffinity purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Neurturin
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 178 to 193 of human neurturin (1).
