The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP).
IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB (1), which is a pivotal transcription factor mediating inflammatory and immune response.
A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (2).
Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation.
IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (3,4).
Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory responses to cytokine IL-1 and LPS.
IRAK2 is expressed in a variety of human tissues.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control: K562 whole cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Supplied as chromatographically purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human IRAK2 (CT). 65kD band can be detected. No cross response to IRAK.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 571 to 590 of human IRAK2 (2).
