Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases.
Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis.
Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 for DAP kinase-related apoptosis-inducing protein kinases (1).
DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain.
Overexpression of DRAK1 induces apoptosis.
DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities.
DRAK1 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues (1).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control: A431 or MOLT4 whole cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Immunoaffinity purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human, mouse and rat DRAK1 (NT). ~50kD band can be detected. No cross responses to DRAK2, DAP or ZIP kinases.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to amino acids 5 to 19 of human DRAK1 (1).
