Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.
These death signals finally cause the degradation of chromosomal DNA by activated DNase.
A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease (1,2).
The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively, (3-5).
DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6) and forms complex with DFF40/CAD.
Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei.
Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Positive Control: K562 or Jurkat whole cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Supplied as affinity chromatography purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human DFF40 (NT) CPAN, hCAD. 40kD band can be detected.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 3 to 18 of human DFF40
