84-1015-59　DFF40, IN (CAD I18, DNA Fragmentation Factor, Caspase Activated Deoxyribonuclease, CPAN) 100ug　D3224-32

特徴

• Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
• Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.
• These death signals finally cause the degradation of chromosomal DNA by activated DNase.
• A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease (1,2).
• The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively, (3-5).
• DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6) and forms complex with DFF40/CAD.
• Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei.
• Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
• SOURCE: Rabbit anti-DFF40 (IN) polyclonal antibody was raised against a peptide corresponding to amino acids 203 to 218 of human DFF40 (4, 5).
• Applications:Suitable for use in ELISA, Immunocytochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control: K562 or Jurkat whole cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.