84-1015-58　DcR1 (TRAIL-R3, TRID, LIT, TRAIL Decoy Receptor1) 100ug　D1499

特徴

• Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors.
• TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines.
• DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3).
• Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT (2-7) and DcR2/TRAIL-R4/ TRUNDD (8-10).
• DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain.
• It is a glycophospholipid-anchored cell surface protein.
• DcR1 transcripts were expressed in many normal human tissues.
• But not in most cancer cell lines (2,3) Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis (2,3).
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blotting: 1:500-1:1000.
• An ~65kD band can be detected (6).
• Optimal dilutions to be determined by the researcher.
• Positive Control: Whole cell lysate from HeLa cells
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.