Apoptosis is related to many diseases and induced by a family of cell death receptors.
Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases.
These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD (1-4).
DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified (5).
CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD (5,6).
Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45.
CIDE-A is expressed in many tissues.
This polyclonal antibody can be used for detection of CIDE-A by Western blot at 1:1000 to 1:2000 dilution.
Human brain tissue lysate can be used as positive control and an approximately 23kD band can be detected.
It has no crossreactivity to CIDE-B.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Immunoaffinity purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human CIDE-A (CT)
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 200 to 217 of human CIDE-A (5).
