84-1015-54　［取扱停止］Bnip3L (IN) Bnip3, Nix 100ug　B2200

特徴

• Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death.
• Bcl-2 homology 3 (BH3) domain is a potent death domain.
• BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family.
• A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (1-3).
• Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3.
• Overexpression of Bnip3L induces apoptosis (2,3).
• Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-XL.
• Bnip3L is localized in mitochondria.
• The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1,2).
• Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-1ug/ml.
• A ~40kD top band represents the monomer of Bnip3L
• Optimal dilutions to be determined by the researcher.
• Related Products:Positive Control: K562 cell lysate; See Cat# K0001-25
• Blocking Peptide: Available for competition studies; See Cat# B2200P.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.