Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.
The mammalian homologous of the key cell death gene CED-4 in C.
elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1 (1,2).
Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation.
Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3).
Apaf1 can also associate with caspase-4 and caspase-8 (4).
Apaf1 transcript is ubiquitously expressed in human tissues (1).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilution determined by the researcher.
Positive Control:Human heart tissue lysate; 130kD band should be detected.
Related Products:Blocking peptide (A2298-64P, 50ug/250ul) is available for competition studies.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Apaf-1 at 130kD. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to amino acids 1,158 to 1,177 of human Apaf1. The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid.
