Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation.
These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1).
A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2).
Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria.
AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection.
AIF is highly conserved between human and mouse and widely expressed (2).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control: K562 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human and mouse AIF (NT) a 67kD band should be detected.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to aa109 -122 of human AIF at N-terminal.
