Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:20,000-1:100,00Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography followed by solid phase adsorption.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish peroxidase (HRP).
Specificity：Recognizes rabbit IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rabbit IgG and rabbit serum. No reaction was observed against bovine, chicken, goat, guinea pig, hamster, equine, human, mouse, rat or sheep serum proteins.
