Deoxyribonuclease I (DNase I) is a Ca2+ and Mg2+ dependent endonuclease.
DNase I is synthesized in the pancreas and stored in zymogen granules.
It has been used to reduce the viscosity of cystic fibrosis sputum.
A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis.
A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:5000-1:20,000.
Assayed against 1ug of Deoxyribonuclease I a standard capture ELISA using Streptavidin (HRP) and ABTS as a substrate for 30 minutes at RT
Western Blot: 1:500-1:5000Immunoprecipitation: 1:100Optimal dilutions to be detemined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：bovine
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 100ul sterile ddH2O. Labeled with Biotin.
Specificity：Recognizes bovine Deoxyribonuclease I. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Deoxyribonuclease I (bovine pancreas).
