Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M.
Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a tetramer of 36kDa subunits.
Every LDH molecule consists of four subunits where each subunit is either H or M.
There are then, five LDHisotypes: LDH-1 (4H) found in heart; LDH-2 (3H1M) found in reticuloendothelial system; LDH-3 (2H2M) found in lung; LDH-4 (1H3M) in kidney and LDH-5 (4M) found in liver and striated muscle.
Usually LDH-2 is the predominant form in serum.
An LDH-1 level higher than LDH-2 suggests damage to heart tissues.
LDH is a cytoplasmic enzyme as determined by Western Blot.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：rabbit
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes rabbit Lactate Dehydrogenase. Assay by IEP resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified Lactate Dehydrogenase [rabbit muscle]. BLAST analysis was perform to determine that crossreactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B)from most mammalian species.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ma Rb
Immunogen：Full length Lactate Dehydrogenase (LDH) from rabbit muscle.
