Apolipoprotein B plays an essential role in lipid transport and metabolism.
Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG CoA reductase.
This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis.
Apo B may be important in the genesis of atherosclerosis.
Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma.
Increased levels of apo B100 are associated with an increased risk of coronary artery disease.
Apo B exists in human plasma as two isoforms: apo B48 and apo B100.
Apo B100 is the major physiological ligand for the LDL receptor.
It contains 4536aa residues.
Apo B100 is the full length protein.
Its gene has been mapped on the short arm of chromosome 2, with an ~length of 43kb and 29 exons.
Apo B100 is synthesised in the liver.
It is required for the assembly of very low density lipoproteins (VLDL).
It does not interchange between lipoprotein particles, as do the other lipoproteins.
It is found in IDL and LDL particles after the removal of the apolipoproteins A, E and C.
Apo B48 is present in chylomicrons and chylomicron remnants.
It plays an essential role in the intestinal absorption of dietary fats.
Apo B48 is synthesized in the small intestine.
It comprises the N-terminal 48% of apo B100.
Apo B48 is the degraded protein.
Apo B48 lacks the LDL receptor-binding domain of Apo B100.
It is produced due to post-transscriptional Apo B100 mRNA editing at codon 2153, which creates a stop codon in the intestine instead of a glutamine in the liver.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Heat inactivated serum.
Form：Supplied as a liquid in 0.02% sodium azide.
Specificity：Recognizes mouse Apo B48 (241kD) and B100 (513kD) as these proteins are highly conserved. Species Crossreactivity: human.
