Applications:Suitable for use in Immunofluorescence and FLISA.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Flourochrome/Protein Ratio: 3.2 moles FITC per mole goat IgG
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2mg
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：lSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes mouse IgG. Monospecific antiserum exhibits single precipitin arc (IEP) against anti-fluorescein, anti-goat serum, mouse IgG and mouse serum. No reaction was observed against human serum proteins.
