Both the antiserum and IgG fractions of anti-carboxypeptidase Y (Baker’s yeast) are shown to detect under reducing conditions of SDS-PAGE the 61kD enzyme in cellular extracts.
Approximately 10ug of total protein is loaded per lane.
A 1:5,000 dilution of the primary antibody is used followed by detection using HRP goat-a-rabbit IgG diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a liquid from PBS, pH 7.2, 0.01% sodium azide
Specificity：Immunoelectrophoretic assay gives a single precipitin arc against anti-rabbit serum, purified carboxypeptidase Y and partially purified carboxypeptidase Y (yeast). Although not tested, the antibody should exhibit crossreactivity with other tissues and species.
