Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (FFPE): 1-2ug/ml with an overnight incubation at 4ºC.
An AP-labeled polymer detection system was used with a red chromogen.
Heat induced antigen retrieval with sodium citrate, pH 6, is reccommended
ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution in conjunction with biotinylated anti-human PF4 (P3360A) as a detection antibody, allows the detection of at least 2000-4000pg/ml of recombinant human PF4Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human PF4 is 1.5-3ng/lane under reducing or non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Country of Origin:USA
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Specificity：Recognizes human PF4 (Platelet Factor 4).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human PF4 derived from E. coli.
