84-1015-14　Macrophage Inflammatory Protein 1 alpha (MIP-1 alpha, CCL3, LD78 alpha) 100ug　M1202-30A

特徴

• Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines.
• They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g.
• macrophages, lymphocytes and NK cells.
• While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes.
• Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells.
• Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells.
• Applications:Suitable for use in ELISA, Western Blot and Neutralization.
• Other applications not tested.
• Recommended Dilution:ELISA (Sandwich): ≥ 0.5-2ug/ml using 100ul/well antibody solution.
• Detects 0.2-0.4ng/well of recombinant rat MIP-1a
• Western Blot: 0.1-0.2ug/ml.
• The dectection limit is 1.5-3ng/lane, under reducing or non-reducing conditions
• Neutralization: 8-10ug/ml yields one-half maximal inhibition (ND50) of the biological activity of rat MIP-1a (100ng/ml)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.