Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases.
Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis.
Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1).
DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain.
Overexpression of DRAK2 induces apoptosis.
DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities.
DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues (1).
Western blot analysis of DRAK2 in Jurkat (1,3) and Raji (2,4) cell lysate in the absence (1,2) or presence (3,4) of blocking peptide with anti-DRAK2 (CT) at 1:500 dilution.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control: Whole cell lysate from Jurkat cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：~45kD band can be detected. It has no cross responses to DAP or ZIP kinases. The ~70kD band is probably non-related to DRAK2 although it is peptide blockable.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acids 351 to 365 of human DRAK2 (1).
