Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas.
Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified.
Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors.
A novel TNF-R1 interacting protein was recently identified and designated SODD for silencer of death domains (1).
SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling.
TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways.
SODD also interacts with DR3.
SODD is ubiquitously expressed in human tissues and cell lines.
Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysate in the absence (1,2) or presence (3,4) of blocking peptide with anti-SODD (NT) at 1:500 dilution.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Positive Control: HeLa and THP-1 whole cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：An approximately 60kD band can be detected. It is human, mouse and rat reactive.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to amino acids 2 to 16 of human SODD (1).
