84-1014-86　DFF40 (CAD, DNA Fragmentation Factor, Caspase Activated Deoxyribonuclease, CPAN) 100ug　D3224-30

特徴

• Apoptosis, the process of programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms and involves dramatic changes in cellular structure.
• Disruption of apoptosis may contribute to cancer and to autoimmune and degenerative diseases.
• Apoptosis is induced by a family of cell death receptors and their corresponding ligands.
• Cell death signals are then transduced by adapter molecules and members of the caspase (ICE/CED-3) family of cysteine proteases that eventually lead to the degradation of chromosomal DNA by activated DNase.
• Recently identified was a mouse DNase that causes apoptotic DNA fragmentation and was designated CAD (for caspase activated deoxyribonuclease) (1,2).
• The human homologue of mouse CAD was recently identified by two groups independently and termed CPAN (caspase activated nuclease) and DFF40 (DNA fragmentation fragment 40) (3,4).
• Human DFF45 and its mouse homologue ICAD are the inhibitors of DFF40 and CAD, respectively (5).
• DFF is composed of two subunits, DFF40/CAD and DFF45/ICAD.
• Cleavage of DFF45/ICAD, which is mediated by caspase-3, leads to DFF40/CADís activation as a nuclease (1–4).
• Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
• DFF40 mRNA is expressed in limited number of human tissues; pancreas, spleen, prostate, and ovary (6).
• Applications:Suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5ug/ml Immunocytochemistry: 5ug/mlOptimal dilutions to be determined by researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.