Human immunodeficiency virus (HIV) and related viruses require coreceptors to infect target cells.
Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors.
The genes encoding human and monkey GPR15/BOB (for G protein-coupled receptor 15 and brother of Bonzo, respectively,) were recently cloned (1,2).
This novel G protein-coupled receptor serves as corecptor for simian immunodeficiency virus (SIV), and for strains of HIV-2 and M-tropic HIV-1 (2,3).
The ligand for GPR15 has not been identified yet.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Positive Control: Jurkat or A549 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：An approximately 50kD band can be detected. It is human, mouse and rat reactive.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to amino acids 13 to 28 of human GPR15 (1). The sequence differs from those of African green monkey and pig-tailed macaque BOB by one amino acid (2)
