Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family.
A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch41,2 and renamed as caspase-10.
Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors.
Caspase-10 is therefore involved in the CD95 and TNFR1 induced apoptosis1.
Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP.
Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death (3).
Caspase-10 is expressed in many tissues and cell lines (1,2).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Controls:HeLa Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Caspase-10, a ~59kD protein, corresponding to the apparent molecular mass of caspase-10/b on SDS-PAGE immunoblots, in samples from human, mouse, rat and bovine. An additional band at 40kD is observed in rat and mouse tissue extracts. Does not recognize caspase-10/a (Mch4).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa505-521 of human Caspase-10.
