84-1014-82　［取扱停止］CIDE-B, CT (Cell Death-inducing DFF-like Effector B) 100ug　C5072-50

特徴

• Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
• Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.
• These death signals finally cause the degradation of chromosomal DNA by activated DNase.
• DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD.
• DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified (1).
• CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD (1,2).
• Expression of CIDE-B induces apoptosis, which is inhibited by DFF45.
• CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation.
• CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes, and bone marrow (1).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Positive Control: Tissue lysate of murine liver
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.