Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases.
Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis.
A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase.
ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family.
ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon.
Overexpression of ZIP kinase induces apoptosis.
ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities.
The messenger RNA was ubiquitously expressed in various tissues.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunocytochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat or HeLa whole cell lysate.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.
Specificity：Recognizes human ZIPK, IN (Zip Kinase). A 52kD band should be detected. Does not crossreact with DAP kinase. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to amino acids near the center of human ZIP Kinase.
