Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.
The mammalian homologues of the key cell death gene CED-4 in C.
elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2).
Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation.
Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3).
Apaf1 can also associate with caspase-4 and caspase-8 (4).
Apaf1 is ubiquitously expressed in human tissues (1).
See corresponding Blocking Peptide for A2298-65P
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000.
50ug of whole cell lysate from HeLa cells with anti-Apaf1 at 1:1000Optimal dilutions to be determined by the researcher.
Positive Control: Whole cell lysate from HeLa cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：A 130kD band should be detected. It is human and mouse reactive.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to amino acids 12 to 28 of human Apaf1 (1). The sequences of the immunogenic peptide are identical between human and mouse (1,2)
